菌种详情

• ZKCC编号：ZKCC21616

• 中文名称：Coriobacterium glomerans

• 拉丁名称：Coriobacterium glomerans

• 模式菌株：是

• 其它保藏中心编号：

• 来源历史：

• 收藏时间：

• 特征特性：

• 参考用途：

• 生物危害程度：一级

• 致病对象：

• 培养基：Clostridium thermocellum medium (ATCC medium 1191)

• 参照ATCC Medium 1190，并用18.75g滤纸取代葡萄糖。若是配试管培养基，可以在每根试管中放入8*70 mm的滤纸条。
  

  ATCC Medium 1190配方:
  

  KH2PO4                            1.5g/L

  Na2HPO4·12H2O              4.2g/L

  NH4Cl                                0.5g/L

  MgCl2·6H2O                      0.18g/L

  酵母提取物                          2g/L

  葡萄糖                                 8g/L

  维生素组分(见下)                 0.5ml/L

  Wolfe's 改良矿物试剂(见下) 5ml/L

  刃天青 (0.1%)                    1ml/L

  Reducing Solution (见下) 40ml/L

  蒸馏水 1L

  注意：This medium contains sodium sulfide, and hydrogen sulfide production will occur, especially upon prolonged boiling. Hydrogen sulfide is hazardous and preparation of this medium should be done in a chemical fume hood
  

  Bring the medium to a boil while gassing with a mixture of 95% N2, 5% H2. Keep the solution boiling for several minutes until time the color turns from blue to reddish-pink. Add the reducing solution during continuous gassing and boiling. The pink color should disappear, indicating reduction. Boil briefly for reduction. Using anaerobic (Hungate) technique, dispense the medium in tubes flushed with N2/H2 gas mixture. Stopper the tubes with butyl rubber, secure and autoclave for 15 minutes.

  维生素组分：
  

  Biotin                              20mg

  p-Aminobenzoic acid    50mg

  Folic acid                       20mg

  Pantothenic acid calcium salt 50mg

  Nicotinic acid                 50mg

  Vitamin B12                   1mg

  Thiamine . HCl               5mg

  Pyridoxine hydrochloride 100mg

  Thioctic acid                  50mg

  Riboflavin                      5mg

  Dissolve the vitamins in 500 ml of water. Add 0.05 ml of this solution per 100 ml of medium. Freeze the vitamin solution or store in a refrigerator in the absence of light.

  Reducing Solution：
  

  0.2 N NaOH                     200ml

  Na2S . 9H2O                    2.5g

  L-Cysteine . HCl               2.5g

  Bring the sodium hydroxide solution to a boil and bubble with 95% N2, 5% H2 gas mixture. Allow the solution to cool and add the sodium sulfide and cysteine. Under anaerobic conditions, tube the solution, stopper with butyl rubber, and autoclave for 15 minutes.
  

  Wolfe's Modified Mineral Elixir:
  

  Nitrilotriacetic acid          1.5g

  MgSO4 . 7H2O                 3g

  MnSO4 . H2O                    500mg

  NaCl                                  1g

  FeSO4 . 7H2O                   100mg

  Co(NO3)2 . 6H2O             100mg

  CaCl2 (anhydrous)            100mg

  ZnSO4 . 7H2O                   100mg

  CuSO4 . 5H2O                   10mg

  AlK(SO4)2 (anhydrous)     10mg

  Boric acid                          10mg
  

  Na2MoO4 . 2H2O             10mg
  

  Na2SeO3 (anhydrous)       1mg
  

  Distilled water                   1L
  

  Suspend the nitrilotriacetic acid in 500 ml water. Dissolve it by titrating with 2-3 N KOH until the pH is stabilized at 6.5. Add the rest of the ingredients and dissolve in the order they are listed. Finally, adjust the volume to 1.0 L For 100 ml of medium, use 0.5 ml of the mineral elixir.

• 培养温度：37℃

• 需氧类型：专性厌氧

• 培养时间：1-2天