菌种详情
ZKCC编号:ZKCC23077
中文名称:Clostridium uliginosum
拉丁名称:Clostridium uliginosum
模式菌株:是
其它保藏中心编号:
来源历史:
收藏时间:
特征特性:
参考用途:
生物危害程度:一级
致病对象:
培养基:DSM Medium 869 with strain-specific modifications
Trypticase soy broth 5.5g
Resazurin (0.1% w/v) 0.5ml
D-Glucose 2g
Distilled water 1000ml
Dissolve dehydrated TSB medium and resazurin, bring to the boil and cool to room temperature while sparging with 100% N2 gas. Adjust pH to 5.5, dispense under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add glucose from a separately sterilized anoxic stock solution.
For DSM 12992: Adjust pH of complete medium to 6.5
培养温度:37℃
需氧类型:专性厌氧
培养时间:4-5天