菌种详情
ZKCC编号:ZKCC23755
中文名称:Coprococcus eutactus
拉丁名称:Coprococcus eutactus
模式菌株:是
其它保藏中心编号:
来源历史:
收藏时间:
特征特性:
参考用途:
生物危害程度:一级
致病对象:
培养基:YCFA
Tryptone 10g
Yeast extract 2.5g
NaHCO3 4g
Glucose 2g
Maltose 2g
Cellobiose 2g
L-Cysteine·HCl·H2O 1g
Mineral solution I 150ml
Mineral solution II 150ml
Resazurin 1mg
VFA mix 6.2ml
Hemin solution 10ml
Vitamin solution I 1ml
Distilled water 660ml
pH adjustment 7.45
After autoclaving, add filter-sterilized 1.0 ml Vitamin solution II (see below).
Mineral solution I:
K2HPO4 3g
Water 1L
Mineral solution II:
KH2PO4 3g
(NH4)2SO4 6g
NaCl 6g
MgSO4·7H2O 0.6g
CaCl2·2H2O 0.6g
Water 1L
VFA mix:
Acetic acid 17ml
Propionic acid 6ml
n-Valeric acid 1ml
iso-Valeric acid 1ml
iso-Butyric acid 1ml
10 N NaOH 26ml
Hemin solution:
KOH 0.28g
Ethanol 25ml
Hemin 0.1g
Water to 100 ml
Vitamin solution I:
Biotin 5mg
Vitamin B12 5mg
p-Aminobenzoic acid 15mg
Folic acid 25mg
Pyridoxine·HCl 75mg
Water 500ml
培养温度:37℃
需氧类型:专性厌氧
培养时间:1-2天