菌种详情

• ZKCC编号：ZKCC25998

• 中文名称：纽约杜博西氏菌

• 拉丁名称：Dubosiella newyorkensis

• 模式菌株：是

• 其它保藏中心编号：

• 来源历史：

• 收藏时间：

• 特征特性：

• 参考用途：

• 生物危害程度：一级

• 致病对象：

• 培养基：DSM Medium 110： CHOPPED MEAT MEDIUM WITH CARBOHYDRATES

• Ground beef (fat free) 500g

  Distilled water 1000ml

  NaOH 1 N 25ml

  Use lean beef or horse meat. Remove fat and connective tissue before grinding. Mix meat, water and NaOH, then boil for 15 min with stirring. Cool to room temperature, skim fat off surface, and filter, retaining both meat particles and filtrate. To the filtrate add water to a final volume of 1000 ml, and then add:

  Casitone 30g

  Yeast extract 5g

  K2HPO4 5g

  Na-resazurin solution (0.1% w/v) 0.5ml

  D-Glucose 4g

  Cellobiose 1g

  Maltose 1g

  Starch (soluble) 1g

  To make medium anoxic boil it, cool under 100% N2 gas atmosphere, add 0.5 g/l Lcysteine hydrochloride and adjust pH to 7.0. Dispense under same gas atmosphere 7 ml medium into Hungate-type tubes (for strains demanding meat particles put those first into the tube (use 1 part meat particles to 4 or 5 parts fluid)). Autoclave at 121C for 30 min. For agar slants use 15.0 g agar per 1000.0 ml medium.

  In some cases (as indicated in the catalogue) the addition of Haemin and Vitamin K1 or Vitamin K3 is necessary. Add to 1000.0 ml of medium after autoclaving:

  Haemin solution (see below) 10ml

  Vitamin K1 or Vitamin K3 solution (see below) 10ml

  Haemin solution:

  Dissolve 50 mg haemin in 1 ml 1 N NaOH; make up to 100 ml with distilled water and filter sterilize. Store refrigerated.

  Vitamin K1 solution:

  Dissolve 0.1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle.

  Vitamin K3 solution:

  Dissolve 5 mg/ml of vitamin K3 in 95% ethanol, dilute to 0.05 mg/ml in water and filter sterilize. Store refrigerated in a brown bottle.

  For DSM 22787 supplement medium after autoclaving with 50 ml/l of anoxic sterile horse serum.

  For DSM 100320 adjust pH of medium to 7.5–8.0.

  For DSM 103060 adjust pH of complete medium to 7.5.

• 培养温度：37℃

• 需氧类型：专性厌氧

• 培养时间：2-3天