菌种详情
ZKCC编号:ZKCC17036
中文名称:Myxococcus fulvus
拉丁名称:Myxococcus fulvus
模式菌株:否
其它保藏中心编号:
来源历史:
收藏时间:
特征特性:
参考用途:
生物危害程度:
致病对象:
培养基:VY/2 AGAR
Baker's yeast 5g
Vitamin B12 0.5mg
CaCl2 x 2 H2O 1.36g
Agar (Difco) 15g
Distilled water 1000ml
Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50°C (use pH-indicator paper). For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton 3.0 g; calciumchloride dihydrate 0.7 g; magnesiumsulphate heptahydrate 2.0 g) to the vial with freeze dried material.
For DSM 11116 reduce amount of vitamin B12 to 0.05 mg/l.
培养温度:28℃
需氧类型:需氧
培养时间:7天