菌种详情

• ZKCC编号：ZKCC17038

• 中文名称：Corallococcus macrosporus

• 拉丁名称：Corallococcus macrosporus

• 模式菌株：否

• 其它保藏中心编号：

• 来源历史：

• 收藏时间：

• 特征特性：

• 参考用途：

• 生物危害程度：

• 致病对象：

• 培养基：VY/2 AGAR

• Baker's yeast                            5g

  Vitamin B12                             0.5mg

  CaCl2 x 2 H2O                         1.36g

  Agar (Difco)                             15g

  Distilled water                          1000ml

  Sterilize vitamin B12 separately by filtration. Prepare and store yeast cells as autoclaved stock suspension (5 g baker's yeast/100 ml distilled water, adjust pH to 6.5 and autoclave). Adjust pH of medium to 7.2 with KOH before, and after autoclaving and cooling to 50°C (use pH-indicator paper). For suspension of freeze-dried cells from ampoules add about 0.5 - 1.0 ml medium MD1 (per liter: casiton 3.0 g; calciumchloride dihydrate 0.7 g; magnesiumsulphate heptahydrate 2.0 g) to the vial with freeze dried material.

  For DSM 11116 reduce amount of vitamin B12 to 0.05 mg/l.

• 培养温度：28℃

• 需氧类型：需氧

• 培养时间：7天