菌种详情

• ZKCC编号：ZKCC33836

• 中文名称：Proteiniphilum propionicum

• 拉丁名称：Proteiniphilum propionicum

• 模式菌株：是

• 其它保藏中心编号：

• 来源历史：

• 收藏时间：

• 特征特性：

• 参考用途：

• 生物危害程度：一级

• 致病对象：

• 培养基：Medium 104 + haemin + Vit K1

• 104c: PY + X MEDIUM (N2/CO2)

  Trypticase peptone (BD BBL)        5g

  Meat peptone (pepsin-digested)   5g

  Yeast extract                                  10g

  Salt solution                                  40ml

  6Sodium resazurin (0.1% w/v)      0.5ml

  L-Cysteine HCl x H2O                    0.5g

  Na2CO3                                         1g

  D-Glucose                                      5g

  Distilled water                                960ml

  Dissolve ingredients (except cysteine, carbonate and glucose) and sparge medium with

  80% N2 and 20% CO2 gas mixture for 30 - 45 min to make it anoxic. Add cysteine, then

  dispense under 80% N2 and 20% CO2 gas atmosphere into anoxic Hungate-type tubes or

  serum vials and autoclave. Add glucose from a sterile anoxic stock solution prepared

  under 100% N2 gas atmosphere and carbonate from a sterile anoxic stock solution

  prepared under 80% N2 and 20% CO2 gas atmosphere. Adjust pH of complete medium to

  7.0, if necessary.

• 培养温度：37℃

• 需氧类型：专性厌氧

• 培养时间：1-2天