菌种详情
ZKCC编号:ZKCC23948
中文名称:Rhodobaca barguzinensis
拉丁名称:Rhodobaca barguzinensis
模式菌株:是
其它保藏中心编号:
来源历史:
收藏时间:
特征特性:
参考用途:
生物危害程度:BSL 1
致病对象:
培养基:RHODOBACA BARGUZINENSIS MEDIUM
KH2PO4 0.5g
NaCl 20g
NaNO3 0.4g
NH4Cl 0.4g
Na2SO4 0.5g
MgCL2 x 6 H2O 0.2g
Yeast extract 1g
Na2SO3 x 5 H2O 1g
KCl 0.5g
Na2CO3 10g
NaHCO3 5g
Trace element solution SL7 1ml
Vitamin B12 (20mg/l) 0.5ml
Distilled water 1000ml
Adjust pH to 9.0-9.5
Trace element solution SL7:
HCl (25%, v/v) 1ml
ZnCl2 70mg
MnCl2 x 4 H2O 100mg
H3BO3 60mg
CoCl2 x 6 H2O 200mg
CuCl2 x 2 H2O 20mg
NiCl2 x 6 H2O 20mg
Na2MoO4 x 2 40mg
Distilled water 1000ml
Prepare and boil the medium in the absence of Na2S2O3 x 5 H2O, NaHCO3, Vitamin B12, MgCl2 x 6 H2O. Cool under a stream of nitrogen and add the remaining components, except vitamin B12 and Na2S2O3 x 5 H2O. Dispense into tubes or bottle fitted with rubber stoppers and autoclave. Add the vitamin B12 (filter sterilized) and Na2S2O3 x 5 H2O from sterile stock solutions.
培养温度:fluorescent light, 2000 lux, 28°C
需氧类型:好氧;光照
培养时间:2-3天